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New Hampshire Department of Environmental Services

Regional Application of an Alternative HPLC Laboratory Method to Detect PSP Toxin

Contract Title: Red Tide Disaster Assistance MOA with DHHS
Grantee: New Hampshire Department of Health and Human Services
Award Period: January 13, 2010-June 30, 2010; end-date extended to 6/30/11 following 7/14/10 Governor and Council approval

Original Proposal

This project was to provide limited side-by-side comparisons of the mouse bioassay/HPLC data from the AOAC method utilized in the project entitled "Regional Advancement of Emerging Methods to Detect PSP Toxin" with a new, simpler HPLC method currently under development at the New Hampshire Department of Health and Human Services (NHDHHS) Public Health Laboratory and the University of New Hampshire (UNH). Project end date was June 30, 2010.

Project Summary

The University of New Hampshire (UNH) and New Hampshire Department of Health and Human Services (NHDHHS) convened a working group to review method development and laboratory results on December 17, 2009, and Chris Nash attended. A Memorandum of Agreement (MOA) between NHDES and NHDHHS was approved by the Governor and Executive Council on January 13, 2010. A no-cost time extension to the Memorandum of Agreement was approved by Governor and Executive Council on July 15, 2010, extending the contract through June 30, 2011. The extension was needed due to a lack of toxic samples from the 2010 PSP season, personnel changes at NHDHHS, and High Pressure Liquid Chromatography (HPLC) equipment issues. On September 13, 2010, 150 shellfish homogenate samples with a wide range of toxicity levels from the Maine Department of Marine Resources (MEDMR) were delivered to the NHDHHS laboratory for analysis. The 150 homogenate samples were split with the NHDES lab for comparison analysis between both alternative HPLC methods and the mouse bioassay (MBA) test.

On June 30, 2011, the extended contract with NHDHHS expired without completion of HPLC sample analysis. Unresolved issues pertaining to the service agreement between NHDHHS and the manufacturer for the HPLC machine were the main cause for the incompletion of tasks under the agreement. In September 2011, after the contract had expired, the issues surrounding the service agreement were resolved and the HPLC machine was repaired. In consultation with our NMFS project officer, NHDES and NHDHHS agreed to not pursue another agreement to complete the work with 2008 Red Tide Disaster Relief funding. Rather, the NHDHHS lab planned to complete the work using funds from sources outside of the red tide grant. Of the original $35,307 budgeted, $20,925.25 was expended. Most of this amount was spent at the projectís outset on a maintenance contract for the HPLC machine. The $14,381.75 in unspent funds was reprogrammed into other projects related to the 2008 Red Tide Disaster Relief grant.

Reports and Products

  • None

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